P8.04 – A unifying, spectrum-centric approach for the analysis of peptide tandem mass spectra
Mass spectrometry-based proteomics data is acquired using data dependent (DDA), data independent (DIA) or targeted acquisition (PRM) methods. Typically, the former is analyzed using spectrum-centric algorithms assuming that it generates non-chimeric spectra, while the latter two are analyzed in a peptide-centric fashion. However, peptide-centric approaches often struggle to control for the contribution of multiple peptides to the experimental fragment ion intensity. Recently, we showed that DDA, and by extension PRM, spectra can be substantially chimeric and introduced an approach that deconvolutes spectra irrespective of isolation window size, thereby substantially boosting the number of identified peptides for DDA. Here, we demonstrate that the same approach generalizes to any chimeric MS2 spectrum, unifying the analysis of DDA, DIA and PRM data.
Date: Tuesday, March 7th
Location: The Drake Hotel in Chicago
Session: Poster Session 2 – 10:00 AM - 11:30 AM
